Hasaacas' nine-game unbeaten run was ended on Wednesday when they tasted their first league defeat in the Division One League.
The Zone 2B leaders were beaten 1-0 by Elmina Sharks in Cape Coast.
Despite the loss, Hasaacas remain six points clear at the top of the table.
In Zone 1A, Yassin FC moved four points clear at the top after defeating Toende United 3-0.
Great Olympics slipped back to the foot of the table in Zone 3A following their 2-1 loss at home to Istanbul.
Full results after match-day 10
Zone 1A
Yassin 3-0 Toende United
Utrecht 0-0 Galazy
Guan United 2-1 Bazooka
Zone 1B
Unity FC 1-0 Bofoakwa
Universal 1-0 B.A. Stars
Berlin 2-0 B.A. United
Bechem United 3-0 Sampa
Zone 2A
KFC Samosa 4-1 Mine Stars
Dunkwa United 2-0 Gold Stars
Deportivo 2-0 Starke FC
Tarkwa United 1-0 Rainbow Stars
Zone 2B
Elmina Sharks 1-0 Hasaacas
Windy Professionals 1-0 Eleven Wise
Metro Stars 1-0 Ajax FC
Brighton stars 2 -0 Wassaman United
Zone 3A
Olympics 1-2 Istanbul
Rehoboth 0-0 Inter Allies
Sporting Mirren 2-0 Prestige
Zone 3B
King Solomon 1-2 Danbort
Okwawu 2-0 Mighty Jets
Zaytuna FC 1-0 Red Bull
Unity Stars 1-1 Pure Joy