Ghana's perfect start to their bid for place at the World Cup in 2010 came to an end in Libreville on Saturday when Gabon chalked a rare 2-0 win over their counterparts.
Roguy Mere opened the scoring in the 42nd minute, latching on to a pass from Eric Mouloungi. France-based Stephane Nguema scored the second in the 66th minute.
It was Gabon's first win of the qualifiers after they lost their opening match in Libya last week. Ghana, who competed at the last World Cup finals in Germany two years ago, remain top of the standings with six
The two sides meet next week in Ghana.
The other two teams in group 5, Lesotho and Libya, play in Bloemfontein on Sunday.
The game between Gabon and Lesotho, which should have kicked-off this campaign, has still to be played.